Dysregulation of Pulpitis Progression Through Modulation of the lncRNA ITGB2-AS1 /miR-1224-5p Axis.

ABSTRACT:

Introduction and aims

The onset of pulpitis is excruciatingly painful, and delayed treatment can lead to serious sequelae. LncRNAITGB2-AS1 is abnormally expressed in pulpitis samples, and thus, the present study was designed to investigate its diagnostic value and regulatory mechanisms in pulpitis.

Methods

RT-qPCR was used to detect the expression of ITGB2-AS1 and miR-1224-5p in 85 cases of pulp tissue and 80 cases of normal tissue. ROC curves were evaluated for the diagnostic value of ITGB2-AS1. Cell proliferation and apoptosis were assayed by the CCK-8 kit and apoptosis kit, respectively. IL-6 and TNF-α levels were measured by ELISA; mRNA levels was assessed by RT-qPCR. SOD and MDA levels were determined using corresponding kits. PPI network analysis identified hub genes a

SUBMITTER: Xu J 

PROVIDER: S-EPMC12702111 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature