Gene profiling and serotyping of multidrug-resistant Listeria monocytogenes isolated from humans, animals, and dairy products.

ABSTRACT:

Background

Listeria monocytogenes is a foodborne pathogen of a major public health concern due to its ability to cause severe illness and its increasing antimicrobial resistance. This study aimed to determine the prevalence, antimicrobial susceptibility, serotype distribution, and virulence genes profiles of L. monocytogenes isolated from food products, farm animals, and humans in Egypt.

Methodology

A total of 750 samples were collected, including dairy products, raw meat, animal fecal samples, aborted fetuses, and human stool samples. Isolation and identification were performed according to ISO, (2022) followed by serotyping and molecular characterization of virulence genes (iap, hlyA, and actA). Antimicrobial susceptibility testing was conducted using the disk diffusion me

SUBMITTER: Shaaban SI 

PROVIDER: S-EPMC12702151 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature