A neutrophil-mesangial cell axis promotes glomerular injury in lupus nephritis.

ABSTRACT:

Objectives

Lupus nephritis (LN) is a major complication of systemic lupus erythematosus (SLE), yet the mechanisms linking neutrophil activation to early kidney damage remain elusive. We aimed to elucidate the role of neutrophils and neutrophil extracellular traps (NETs) in driving mesangial cell (MC) activation during LN progression.

Methods

Histology and flow cytometry were performed in NZB/W F₁ and C57/BL6 mice to evaluate neutrophil dynamics throughout LN development. Human LN kidney biopsies were analysed for NETs and neutrophil infiltration. In vitro, human MCs were stimulated with either NETs or purified citrullinated histone H3 (citH3), followed by assessment via enzyme-linked immunosorbent assay and RNA sequencing. Plasma levels of NET remnants (citH3-DNA

SUBMITTER: Hanata N 

PROVIDER: S-EPMC12702198 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature