Microscaled Cell Surface Proteomics for Cryo-Preserved Cells and Tissue Samples.

ABSTRACT: Cell surface proteins (CSPs) regulate key cellular functions and represent valuable targets for diagnostics and therapeutics. Despite advances in proteomic workflows, CSP analysis from cryopreserved or low-input clinical samples remains limited by technical constraints, including reduced membrane integrity, inefficient labeling, and high background. To address these challenges, we optimized and benchmarked two complementary surface enrichment strategies compatible with low-input applications (fewer than 1 million cells) and real-world sample types, including viably cryopreserved cells and dissociated solid tissues. We systematically compared oxidation-based N-glycopeptide capture and wheat germ agglutinin-horseradish peroxidase-mediated proximity labeling across a range of input amounts us

SUBMITTER: Thorup JR 

PROVIDER: S-EPMC12702325 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature