Lactate as a metabolic-epigenetic signal linking high-intensity interval training (HIIT) to miRNA-Centered remodeling of the skeletal muscle methylome and transcriptome.

ABSTRACT:

Background

Lactate, a key exercise-derived metabolite and exerkine, is increasingly recognized as a metabolic-epigenetic signal, yet whether lactate and its transport directly shape skeletal-muscle epigenetic and transcriptional adaptations to exercise remains unclear.

Methods

Young male mice underwent six-week interventions: Control, lactate administration, high intensity interval training (HIIT), monocarboxylate transporter isoforms 1 and 2 (MCT1/2) inhibition, or HIIT plus inhibition. Gastrocnemius muscle was profiled by DNA methylation arrays, mRNA and miRNA-seq, together with analyses of signaling proteins, metabolites, and running performance.

Results

Exogenous lactate and HIIT each elicited broad, site-specific remodeling of the skeletal muscle methylome and tr

SUBMITTER: Zhou L 

PROVIDER: S-EPMC12702345 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature