Sustainable valorization of <i>Pleurotus pulmonarius</i> via green dual-enzyme technology: Novel optimization for functional protein hydrolysates.

ABSTRACT: This research established a sustainable dual-enzyme hydrolysis method for generating nutrient-dense hydrolysates from grey oyster mushrooms (Pleurotus pulmonarius), a native resource of Vietnam. Process optimization integrated a quadratic regression model using response surface methodology (RSM) and an artificial neural network combined with a genetic algorithm (ANN-GA). The objective was to enhance nitrogen recovery, amino acids, peptides, glutamic acid, and lysine, while minimizing NH₃ formation. ANN-GA demonstrated superior predictive accuracy compared to RSM, with RMSE values of 1-2 % versus 2-5 %. In contrast, RSM offered precise quantitative insights regarding the effects of factors. The optimal conditions (50.9 °C, 6.45 h, pH 6.12) resulted in a nitrogen recovery o

SUBMITTER: Giang NTN 

PROVIDER: S-EPMC12702349 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature