Safety and efficacy of AAV8-aflibercept in treating choroidal neovascularization via single-cell RNA sequencing.

ABSTRACT: This study evaluated the efficacy and safety of adeno-associated virus serotype 8 (AAV8)-aflibercept for treating choroidal neovascularization (CNV) in mice model, utilizing single-cell RNA sequencing (scRNA-seq) to investigate retinal effects. AAV8-aflibercept was administered via subretinal injection to mice, followed by laser-induced CNV modeling. Successful expression was achieved following subretinal injection of AAV8-aflibercept, aflibercept protein expression showed a gradual decline after peaking at week 4, but remained at relatively stable levels through weeks 8-24. The therapeutic effect was assessed using fluorescein angiography and quantified. AAV8-aflibercept significantly decreased CNV areas across dosages from 3 × 10⁷ to 1 × 10⁹ GC/eye, with minor retin

SUBMITTER: Shi J 

PROVIDER: S-EPMC12702363 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature