Mitochondrial metabolic remodeling and multi-omics profiling identify plasma biomarkers of myocardial infarction.

ABSTRACT: Mitochondrial dysfunction is a hallmark of myocardial infarction (MI), yet the molecular mechanisms linking metabolic reprogramming in the ischemic myocardium to systemic biomarker signatures remain incompletely understood. In this study, we employed a data-independent acquisition mass spectrometry (SWATH-MS) strategy integrating multi-omics analysis with upstream regulatory network analysis to investigate mitochondrial energy pathway alterations in a preclinical ovine model of MI. Proteomic profiling of infarcted myocardium revealed a pronounced shift from oxidative phosphorylation to glycolysis, accompanied by coordinated suppression of mitochondrial fatty acid β-oxidation enzymes. This metabolic reprogramming was strongly associated with four upstream master regulators, most notably pre

SUBMITTER: Paramasivan S 

PROVIDER: S-EPMC12702414 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature