SNP rs10748643 determines CD39 expression in T and NK cells through altering NFIC binding affinity rather than interfering with RNA splicing.

ABSTRACT: NTPDase-1 CD39, a dominant component of purinergic signaling, has proved to be a critical regulator of tissue microenvironment and cellular function particularly for T cells. We repeatedly use single-nucleotide polymorphism (SNP) rs10748643 genotype as a tag of CD39 expression in T cells in many studies. However, no study that profiles the impact of the SNP mutation on CD39 expression in all immune cells has been done yet. In this study, we used flow cytometry to measure CD39 expression in six types of immune cells across three genotypes. We revealed that SNP rs10748643 mainly determines CD39 expression in T and natural killer (NK) cells. In contrast, it has the least influence on monocytes, B cells, and granulocytes. These immune cells demonstrate constitutive CD39 expression, which is di

SUBMITTER: Gan C 

PROVIDER: S-EPMC12702745 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature