Differential impacts of hemin and free iron on amoxicillin susceptibility in <i>ex vivo</i> gut microbial communities.

ABSTRACT:

Introduction

The rise of antibiotic-resistant infections worldwide has created a need to enhance the efficacy of existing antibiotics. Modification of metabolism has been shown to potentiate antibiotic lethality. In this study, we employed a novel ex vivo microbiome culture approach to study the effects of different forms of iron on amoxicillin susceptibility.

Methods

Synthetic and human stool-derived microbiota were cultured and treated with amoxicillin, with growth monitored by optical density. These samples were sequenced using an Oxford nanopore long-read 16S rRNA V4-V9 approach and computationally defined using the Emu algorithm. The validity of this pipeline was confirmed with consortia, murine cecal content, and a human stool sample. The stool-derived community was th

SUBMITTER: Pagano F 

PROVIDER: S-EPMC12702856 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature