Striatal M₄ muscarinic receptors determine the biological rhythm of activity, with a supportive role of M₁ muscarinic receptors.

ABSTRACT:

Introduction

M₄ muscarinic receptor (mAChR) knockout changed the female activity biological rhythm parameters. In this study, we focus on the biological rhythms of mAChRs (total + M₁ mAChRs), acetylcholinesterase (AChE), and butyrylcholinesterase (BuChE) in M₄ mAChR knockout (M₄KO) and wild-type (WT) mice in specific brain areas.

Methods

Female mice were sacrificed every 4 hours, brains were removed, mAChRs were determined by autoradiography, and punching was used for the measurement of acetylcholinesterase and butyrylcholinesterase activity. The density of mAChRs was correlated with locomotor activity.

Results

An ultradian rhythm in total mAChRs was found in the suprachiasmatic nucleus (SCN) (both M₄KO and WT). M

SUBMITTER: Janisova K 

PROVIDER: S-EPMC12702858 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature