Three dimensional visualization of subcellular and suborganellar structures in near-native plant cells by synchrotron soft X-ray tomography.

ABSTRACT: Significant advances in photosynthesis research have been achieved using reverse genetics alongside cellular imaging techniques such as confocal microscopy and transmission or scanning electron microscopy. However, these conventional imaging methods often require chemical fixation or physical sectioning, which can alter the native endocellular ultrastructure and limit the ability to observe subcellular environments. In this study, we utilized synchrotron-based soft X-ray tomography (SXT) at the TPS-24A1 beamline of the National Synchrotron Radiation Research Center (NSRRC), Taiwan, to non-destructively visualize three-dimensional ultrastructure of protoplasts isolated from Arabidopsis thaliana and Bidens pilosa in their near-native state. Our results revealed marked differenc

SUBMITTER: Hua MD 

PROVIDER: S-EPMC12702899 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature