Upscaling: efficient generation of human lung organoids from induced pluripotent stem cells using a stirring bioreactor.

ABSTRACT:

Introduction

Human induced pluripotent stem cells (iPSCs) can be successfully differentiated into complex (three-dimensional) lung spheroids or organoids and have thus proven to be promising in vitro tools that provide a robust system for simulating lung disease and modeling drug response. We previously described a very simple and practical protocol for producing iPSC-derived lung organoids (iPSC-LuOrgs) in ultra-low attachment plates without relying on a gel-like extracellular matrix.

Methods

Here, we produced these organoids in a stirred-tank bioreactor equipped with a unique membrane stirrer and compared this type of up-scaled and more automated cultivation with the manual variant. Detailed morphological and molecular analyses, including single-cell RNA sequencing, of the

SUBMITTER: Budeus B 

PROVIDER: S-EPMC12702919 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature