Hybrid identity and distinct methylation profiles of incomplete intestinal metaplasia in the stomach.

ABSTRACT:

Background

Gastric intestinal metaplasia (GIM), particularly the incomplete subtype (Inc IM), is strongly associated with increased gastric cancer (GC) risk. However, its role as a true precursor lesion remains uncertain.

Objective

We aimed to delineate the molecular identity, differentiation potential and oncogenic relevance of Inc IM.

Methods

Spatial transcriptomics using a custom lineage-enriched panel was applied to profile GIM and GC tissues. Subtype-specific GIM organoid models were developed for DNA methylation and chromatin accessibility profiling. Single-cell RNA sequencing was performed to evaluate differentiation capacity.

Results

Spatial transcriptomics revealed that Inc IM potentially originates from the deep antral gland cells and harbours a hybri

SUBMITTER: Kim H 

PROVIDER: S-EPMC12703266 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature