Molar-Scale Phenolic Acid Decarboxylation Using Thermostable Biocatalysts and Enzyme-Compatible Deep Eutectic Solvents.

ABSTRACT: The synthesis of biogenic hydroxy styrenes by enzymatic decarboxylation of phenolic acids (e.g., ferulic, p-coumaric, caffeic, or sinapic acids) is a promising route involving biorefineries and biocatalysis, in which the low substrate solubility in water requires the quest for nonconventional media. This work explores the tunability of deep eutectic solvents (DES) to simultaneously create enzyme-compatible and solubilizing systems, in which thermostable phenolic acid decarboxylases (PAD) may operate at 50-70 °C, decreasing the inherent DES viscosity. Four DESs displaying high solubility of ferulic acid are assessed (with additions of up to 20 vol% of phosphate buffer): Choline chloride-glycerol (ChCl-Gly, 1:2), choline chloride-ethylene glycol (ChCl-EG, 1:2), choline acetate-glycerol (ChAc

SUBMITTER: Vaupel S 

PROVIDER: S-EPMC12703459 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature