Behavior of Aspartic Proteases From Artichoke Flower (Cynara cardunculus L. var Scolymus) in the Hydrolysis of Buffalo Milk Casein.

ABSTRACT: The objective of this study was to assess the activity of proteases from artichoke flower extract in the enzymatic hydrolysis of casein from buffalo milk and to investigate its potential application in cheese production. Artichoke flower extract and microbial chymosin were assessed for their proteolytic activity (PA) and milk clotting activity (MCA) under varying conditions of pH, temperature, CaCl₂, and NaCl concentration. The peptides obtained from the PA were analyzed using reverse phase high performance liquid chromatography, while the clots obtained from the MCA were analyzed through electrophoresis and mid-infrared spectroscopy. Mini cheeses were produced under optimal conditions established by the specificity ratio (SR) value to evaluate the technological application of a

SUBMITTER: Onelli RRV 

PROVIDER: S-EPMC12703569 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature