MiR-423-5p-enriched small extracellular vesicles drive periodontal regeneration via Sfrp2+ cell expansion.

ABSTRACT: Small extracellular vesicles (sEVs) show therapeutic potential for periodontitis but functional components remain unclear, limiting clinical periodontal therapy application. Identifying key bioactive molecules and enhancing their functions via engineering strategies may overcome these limitations. By comparing the periodontal tropism of sEVs from different stem cells and conducting functional miRNA profiling, we identified miR-423-5p as a key component for periodontal ligament cell (PDLC) osteogenic differentiation by targeting PLCB1 (Phospholipase C Beta 1). We engineered miR-423-5p-enriched sEVs (sEVs^miR-423-5p) with miRNA loading levels up to 100,000-fold higher than those of native sEVs. Compared with unmodified sEVs, sEVs^miR-423-5p promoted the formation o

SUBMITTER: Ma L 

PROVIDER: S-EPMC12703869 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature