Development of an H9N2 vaccine strain with enhanced neuraminidase immunogenicity via dual hemagglutinin and neuraminidase modification.

ABSTRACT:

Importance

The Y439 lineage 01310 E20 H9N2 vaccine strain currently used in South Korea has undergone extensive egg adaptation, resulting in substantial changes, including an 18-amino acid neuraminidase (NA) stalk deletion. Additionally, both early and late 01310 passages inherently harbor an N-glycan at hemagglutinin (HA) residue 158-160 (HA158) that may interfere with virus-specific antibodies.

Objective

We aimed to develop a high-yield vaccine strain without egg passaging and to overcome the limitations of the conventional vaccine strain that may compromise immunogenicity.

Methods

We introduced a genetically modified 01310 PB2 gene (310-MVV: I66M, I109V, I133V) to increase replication in embryonated eggs and removed the N-glycan at HA158 and restored the NA stalk t

SUBMITTER: Song JH 

PROVIDER: S-EPMC12703918 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature