Isolation and Characterization of Extracellular Vesicles From Ascochyta rabiei, a Necrotrophic Fungal Chickpea Pathogen.

ABSTRACT: Extracellular vesicles produced by pathogenic organisms play a role in host recognition and invasion across kingdoms, including fungi interacting with their plant hosts. Little is known about EVs from phytopathogenic fungi compared to human fungal pathogens. Ascochyta rabiei is an endemic and widespread necrotrophic ascomycete fungus that causes a significant impact on chickpea production. The presence of EVs generated by A. rabiei during the invasion of chickpea has not previously been reported. Here, A. rabiei EVs were identified and isolated from liquid fungal broth cultures, both with and without host, using optimized ultracentrifugation and filtration techniques. Nanoparticle tracking analysis, transmission electron microscopy, and mass spectrometry revealed 40-200 nm cup-shaped, doub

SUBMITTER: Ghaheri M 

PROVIDER: S-EPMC12704033 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature