Whole-genome characterization of prevalent dengue virus serotype-1 in 2023 dengue outbreak of Xishuangbanna, a border area of Laos, Myanmar, and China.

ABSTRACT:

Objectives

From June to November 2023, a severe dengue fever outbreak occurred in Xishuangbanna, China, a border area with Myanmar and Laos. This study aimed to identify the pathogen responsible for this outbreak and analyze the genomic characteristics of epidemic strains, providing reference data for dengue prevention and control.

Methods

Serum samples from dengue virus (DENV) NS1-positive patients (July-October 2023) were collected. RNA extraction, serotyping via probe quantitative polymerase chain reaction, and whole-genome amplification using 18 primer pairs followed by sequencing were performed. Base and amino acid mutations were analyzed using DNAMAN. Phylogenetic trees (maximum likelihood for the whole genome and neighbor-joining for the E protein) were constructed us

SUBMITTER: Peng C 

PROVIDER: S-EPMC12704074 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature