ELOVL3 regulates phospholipid homeostasis and thermogenesis in brown adipose tissue.

ABSTRACT: Nonshivering thermogenesis plays an important role in maintaining body temperature and energy homeostasis. Elongation of very long-chain fatty acid protein 3 (ELOVL3), which catalyzes the synthesis of C20-C24 fatty acids, is induced in brown adipose tissue (BAT) by cold exposure and regarded as a thermogenic gene. However, its precise role in thermogenesis remains elusive. BAT-specific KO mice of Elovl3 were generated by the Cre/LoxP approach and phenotypically analyzed under cold exposure. Gene expression changes in BAT were characterized by quantitative RT-PCR and Western blotting, BAT remodeling was evaluated by histological examination, and lipid composition was examined by lipidomic analysis. BAT-specific deletion of the Elovl3 gene resulted in cold intolerance because of impaired BAT

SUBMITTER: Qin Z 

PROVIDER: S-EPMC12704387 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature