Longitudinal, intra-individual stability of untargeted plasma and cerebrospinal fluid metabolites.

ABSTRACT:

Background/objectives

Longitudinal metabolomics analysis offers valuable insight into how metabolic pathways change according to age and health status. However, metabolite levels can fluctuate due to biological factors (ex. age, diet, health-status) and technical factors (ex. sample handling, storage times, instrument performance), with some metabolites exhibiting greater sensitivity to these sources of variability than others. This study aimed to characterize the longitudinal and technical stability of untargeted plasma and cerebrospinal fluid (CSF) metabolites, and to identify a subset that remains reliable over the extended time scales required for epidemiological research.

Methods

Untargeted ultra-high-performance liquid chromatography-mass spectrometry (LC-MS) metabolom

SUBMITTER: Rocha B 

PROVIDER: S-EPMC12704631 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature