A fuzzy sequencer for rapid DNA fragment counting and genotyping.

ABSTRACT: High-throughput sequencing technologies generate a vast number of DNA sequence reads simultaneously, which are subsequently analysed using the information contained within these fragmented reads. The assessment of sequencing technology relies on information efficiency, which measures the amount of information entropy produced per sequencing reaction cycle. Here we propose a fuzzy sequencing strategy that exhibits information efficiency more than twice that of currently prevailing cyclic reversible terminator sequencing methods. To validate our approach, we develop a fully functional and high-throughput fuzzy sequencer. This sequencer implements an efficient fluorogenic sequencing-by-synthesis chemistry and we test it across various application scenarios, including copy-number variation det

SUBMITTER: Zhou W 

PROVIDER: S-EPMC12705452 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature