B7-H3-liquid biopsy for the characterization and monitoring of the dynamic biology of prostate cancer.

ABSTRACT:

Background

B7-H3 is a promising target for cancer therapy, notably in prostate cancer (PCa), particularly in metastatic, castration-resistant PCa (mCRPC). With the development of B7-H3-targeted therapies, there is a need for a rapid, reliable, and cost-effective method to detect and monitor B7-H3 expression. Leveraging their abundance and stability, we developed a liquid biopsy assay using extracellular vesicles (EVs) for this purpose.

Methods

B7-H3⁺ EVs were isolated using a B7-H3 antibody-mediated, click chemistry-based enrichment method. Antibodies were conjugated to methyltetrazine-grafted microbeads. EVs were isolated from 100 µL of plasma from metastatic, castration-sensitive PCa (mCSPC) (n = 43) and mCRPC (n = 103) patients and quantified using RT-qPCR of A

SUBMITTER: Ju Y 

PROVIDER: S-EPMC12705526 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature