Cell cycle-driven transcriptome maturation confers multilineage competence to cardiopharyngeal progenitors.

ABSTRACT: During development, stem and progenitor cells divide and transition through multipotent states to generate the diverse cell types by undergoing defined changes in biomolecular composition, which underlie the progressive loss of potency and acquisition of lineage-specific characteristics. For example, the cardiac and pharyngeal muscle programs are jointly primed in multipotent cardiopharyngeal progenitors, and segregate in distinct daughter cells only after cell division. Here, using the tunicate Ciona, we showed that multipotent cardiopharyngeal progenitors acquire the competence to produce distinct Tbx1/10 (+) and (-) daughter cells shortly before mitosis, which is necessary for Tbx1/10 activation. By combining transgene-based sample barcoding with single-cell RNA-sequencing (scRNA-seq),

SUBMITTER: Bernadskaya YY 

PROVIDER: S-EPMC12705688 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature