MiRNA Biomarkers Diagnose Amyotrophic Lateral Sclerosis in Circulating Blood.

ABSTRACT: A rapid, accurate diagnostic test for amyotrophic lateral sclerosis (ALS) would reduce diagnostic delays and improve patient outcomes. We extracted eight circulating miRNAs from 788 blood plasma samples and analyzed them using qPCR for ALS diagnostic accuracy. The biomarker parameters were established previously using 449 individual blood samples and applied prospectively to an independent cohort for validation of a predictive model. The primary outcome was ALS classification accuracy as measured by diagnostic sensitivity, specificity, positive and negative predictive values (PPV, NPV), and area under the curve (AUC). The secondary outcome was comparative fold-regulation values determined prior to data collection. The diagnostic test had an AUC of 0.98 (95% CI 0.97-0.99), with 97% sensitiv

SUBMITTER: Dunlop RA 

PROVIDER: S-EPMC12705848 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature