Application of Raman Spectroscopy and Micro-Indentation to Micro-Map the Path and Boundary of NaOCI-Induced Dentine Collagen Changes in an Ex-Vivo Root Canal Irrigation Model.

ABSTRACT:

Objectives

To apply Raman spectroscopy and micro-indentation to micro-map the path and boundary of NaOCl-induced dentine collagen changes in an ex-vivo root canal irrigation model.

Material and methods

Root canals of extracted single-rooted teeth were prepared and irrigated with NaOCl or saline. Four teeth (NaOCl = 3; saline = 1) embedded in epoxy resin and sectioned transversely into discs were Raman-analyzed on coronal surfaces from inter- and intra-tubular dentine in 4 quadrants over 12, 24, and 48 min-acquisition times. Eight additional teeth stratified by root maturity, irrigated with NaOCl (n = 7) or saline (n = 1), sectioned transversely and then embedded, were Raman-analyzed on apical surfaces at 18 equidistant (50 μm) points/quadrant for Amide bands. Micro-indentati

SUBMITTER: Ng Y 

PROVIDER: S-EPMC12705904 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature