Fifty high-content light sheet fluorescence microscopy datasets of Tribolium castaneum embryogenesis.

ABSTRACT: The red flour beetle (Tribolium castaneum) is a key model organism in developmental biology, genetics, and agricultural research. To address the limited availability of high-quality microscopy data documenting its embryonic morphogenesis, we assembled the first Systematic Live Imaging Collection of Embryogenesis (SLICE-1), a large open-access resource for Tribolium embryogenesis. Using light sheet fluorescence microscopy, we acquired fifty high-content datasets totaling 200 days of recording time, 5.7 million images, and 2.7 Terabytes of data. Imaging employed five novel transgenic lines expressing mEmerald-labeled nanobodies that target distinct intracellular structures under control of the consecutively and ubiquitously active tubulin alpha 1-like protein promoter. Each line encompasses

SUBMITTER: Strobl F 

PROVIDER: S-EPMC12705995 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature