Non-cell-autonomous control of mouse gastruloid development by the ultra-conserved lncRNA T-UCstem1.

ABSTRACT: The role of long non-coding RNAs (lncRNAs) in early mammalian embryogenesis remains unclear due to the complexity of the regulatory mechanisms involving lncRNAs and the limited availability of embryo samples. Stem cell-based models, such as mouse gastruloids, provide new ways to address these challenges. Here, we investigate the function of ultra-conserved lncRNA T-UCstem1 in mammalian body plan formation. Combining morphological and immunofluorescence imaging analyses with bulk and single-cell transcriptomics, we provide evidence indicating that T-UCstem1 is important for mouse gastruloid development and anteroposterior axis extension. T-UCstem1 depletion in gastruloids results in their aberrant development, with defects in the expression of differentiation markers and persistence of plur

SUBMITTER: Coppola A 

PROVIDER: S-EPMC12706062 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature