Portable RT-LAMP Platform for Rapid Point-of-Care Detection for SFTSV.

ABSTRACT: Severe Fever with Thrombocytopenia Syndrome virus (SFTSV) is a tick-borne pathogen associated with high mortality, particularly among the elderly, and poses a significant public health threat in East Asia's rural areas. Improved diagnostic approaches are crucial for effective disease management in resource-limited settings. In this study, we developed a portable Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) platform for rapid and accurate SFTSV detection. Primers targeting the conserved L gene were designed using 158 sequences collected between 2019 and 2023, and a multiplex assay incorporating the β-actin gene as an internal control to verify amplification efficiency in alignment with clinical performance requirements. We assessed the assay's sensitivity and speci

SUBMITTER: Lee GC 

PROVIDER: S-EPMC12706147 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature