Oral Administration of <i>Clostridium butyricum</i> Alleviates High-Fat Diet-Induced Obesity in Mice by Modulating Gut <i>Akkermansia muciniphila</i> Abundance via Direct Growth Promotion.

ABSTRACT: This study investigated the anti-obesity effects of Clostridium butyricum (CLB) and the underlying mechanisms through gut microbiota modulation. High-fat diet (HFD)-fed C57BL/6 mice were orally administered CLB (1 × 10¹⁰ CFU/kg/day) for 8 weeks. CLB intervention reduced body weight gain by 13.20% and decreased fat mass (21.10 ± 2.24% vs. HFD: 23.39 ± 2.34%, P < 0.05), while partially restoring fecal butyrate levels (11.73 ± 4.99 vs. HFD: 7.27 ± 3.40 μg/g, P < 0.05). 16S rRNA gene sequencing revealed CLB selectively increased the abundance of Akkermansia, which was depleted in HFD mice. In vitro, butyrate, CLB lysate, and culture supernatant significantly enhanced the growth of Akkermansia muciniphila (AKK), indicating microbial cross-fee

SUBMITTER: Dong X 

PROVIDER: S-EPMC12706150 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature