Disclosing New Insights on Pyrazolo[3,4-<i>d</i>] Pyrimidine Tethered Diverse Amino Acid Candidates as Potential DHFR Inhibitors and Anti-Virulence Agents.

ABSTRACT: The rise of bacterial resistance presents a pressing public health challenge, necessitating innovative antimicrobial solutions. Based on our prior work demonstrating the activity of pyrazolo[3,4-d] pyrimidine-based analogues against human dihydrofolate reductase (DHFR), we hypothesized that novel derivatives could function as dual-action antibacterial agents. In this study, we therefore evaluated the capacity of these compounds to both inhibit bacterial DHFR and disrupt quorum sensing (QS)-mediated virulence. The compounds were screened for antibacterial activity against several bacterial strains and the anti-DHF activity was assayed. Anti-virulence potential was assessed in vitro and in vivo, while interactions with the QS targets were investigated using in silico stu

SUBMITTER: Ibrahim TS 

PROVIDER: S-EPMC12706152 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature