Histone lactylation-mediated up-regulation of IGF2BP2 enhances ferroptosis resistance via Nrf2 in colorectal cancer.

ABSTRACT:

Background

Emerging evidence suggests that ferroptosis resistance may drives colorectal cancer (CRC) pathogenesis and limits therapeutic efficacy. A previous study has reported that lactate can enhance ferroptosis in CRC cells. The objective of this study was to elucidate how lactate regulates ferroptosis in CRC and to identify potential therapeutic targets.

Methods

Cellular viability and proliferative capacities were determined via cell counting kit-8 (CCK-8) and colony formation. Ferroptosis-related and inflammatory markers, including malondialdehyde (MDA), Fe²⁺, glutathione (GSH), IL-1β, IL-12 and IL-10, were quantified by commercial kits. Protein and RNA interactions were investigated using co-immunoprecipitation (Co-IP), RNA pull-down, dual-luciferases report

SUBMITTER: Zhu JF 

PROVIDER: S-EPMC12706171 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature