An improved vitrification protocol for the fast and safe storage of mouse oocytes†.

ABSTRACT: Cryopreservation methods for archiving and distributing mouse strains mostly focus on freezing embryos or sperm. Although protocols for the cryopreservation of wild-type mouse oocytes are available, these methods have not been widely adopted in large biomedical research facilities. This is partly due to a lack of validation of the available methods on a large scale using a range of genetically modified oocytes. Furthermore, some of the existing methods report a relatively low rate of fertilization, requiring either the zona pellucida to be physically breached or the inclusion of cumulus cells to enable efficient fertilization, interventions which might be incompatible with maintaining hygiene barriers. Existing methods also often use cryovials rather than straws or slimline vitrification d

SUBMITTER: Hodgson T 

PROVIDER: S-EPMC12706468 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature