Impact of physiological ionic strength and crowding on kinesin-1 motility.

ABSTRACT: The motility of biological molecular motors has typically been analyzed by in vitro reconstitution systems using motors isolated and purified from organs or expressed in cultured cells. The behavior of biomolecular motors within cells has frequently been reported to be inconsistent with that observed in reconstituted systems in vitro. Although this discrepancy has been attributed to differences in ionic strength and intracellular crowding, understanding how such parameters affect the motility of motors remains challenging. In this report, we investigated the impact of intracellular crowding in vitro on the mechanical properties of kinesin under a high ionic strength that is comparable to the cytoplasm. Initially, we characterized viscosity in a cell by using a kinesin motor lacking the car

SUBMITTER: Sagawa M 

PROVIDER: S-EPMC12706510 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature