Plasma-Microdroplet Fusion for Online Post-Column Epoxidation: Toward Deep Lipidomics on Unmodified Mass Spectrometers.

ABSTRACT: The ability to characterize closely related lipids is clinically important, requiring the development of analytical tools to differentiate species responsible for metabolic disorders from those needed for metabolic homeostasis. Herein, we report a new liquid chromatographic (LC) method that utilizes online microdroplet-based epoxidation reactions during electrospray to enable C═C bond localization on conventional tandem mass spectrometry (MS/MS). Through a coaxial spray mechanism, charged microdroplets derived from the LC column and containing the lipid analyte were fused with nonthermal plasma, which facilitated (i) positive ion mode detection of various lipid classes and (ii) instantaneous C═C bond epoxidation via reaction with reactive oxygen species in the nonthermal plasma. Consequent

SUBMITTER: Panday NK 

PROVIDER: S-EPMC12706754 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature