Myelinated fiber labeling and orientation mapping of the human brain with light-sheet fluorescence microscopy.

ABSTRACT: The convoluted network of myelinated fibers that supports behavior, cognition, and sensory processing in the human brain is the source of its extraordinary complexity. Advancements in tissue optical clearing, 3D fluorescence microscopy, and automated image analysis have enabled unprecedented insights into the architecture of these networks. Here, we investigate the multiscale organization of myelinated fibers in human brain tissue from the brainstem, Broca's area, hippocampus, and primary visual cortex by exploiting a specific fiber staining method, light-sheet fluorescence microscopy (LSFM), and an advanced spatial orientation analysis tool. Using an optimized protocol that integrates tissue clearing with the lipophilic DiD probe to achieve uniform and deep myelinated fiber labeling, we g

SUBMITTER: Sorelli M 

PROVIDER: S-EPMC12706767 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature