Neurotrauma induced retinal basement membrane COL4A1 defects are restored by adipose tissue derived mesenchymal stem cell concentrated conditioned medium.

ABSTRACT:

Background

COL4A1 defects are known to cause a variety of multisystem disorders with significant vascular dysfunction leading to neuronal damage. Case reports suggest that patients with COL4A1 mutations or extracellular COL4A1 deficiency in the basement membrane may put individuals at increased risk for developing visual deficits with neurotrauma. However, no experimental evidence is available. This study investigated the impact of Col4a1 deficiency on visual dysfunction following mild traumatic brain injury (mTBI) and evaluated the therapeutic efficacy of COL4A1-enriched adipose-derived stem cell-conditioned medium (ASC-CCM) in mitigating associated neurovascular deficits.

Methods

Using a retina-targeted knockdown approach in C57Bl/6 mice via intravitreal delivery of AAV2-C

SUBMITTER: Rasiah PK 

PROVIDER: S-EPMC12706880 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature