Laminin-conjugated aligned nanofiber yarns for topographical and biochemical guidance of neurite outgrowth and branching regulation.

ABSTRACT:

Background

Aligned nanofiber scaffolds have shown great promise in promoting neurite orientation and elongation, making them a key design strategy in peripheral nerve tissue engineering. However, most current designs lack biochemical specificity and long-term stability, limiting their regenerative efficacy.

Results

Herein, we developed structurally aligned nanofiber yarns (NFYs) scaffold conjugated with laminin via covalently incorporated N-hydroxysuccinimide (NHS) ester groups. The NHS-functionalized NFYs were fabricated using a dry-wet electrospinning approach based on a copolymer of N-succinimidyl acrylate (AA-NHS) and N-isopropyl acrylamide (NIPAM), blended with polycaprolactone (PCL) and multi-walled carbon nanotubes (MW-CNTs). This scaffold integrates both topographica

SUBMITTER: Jia N 

PROVIDER: S-EPMC12706892 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature