Semiquantitative immunohistochemical (IHC) pixelwise H-score of mitochondrial transcription factor A (TFAM) in gastric adenocarcinoma (GAC): clinicopathological significance and association with p53 and HER2.

ABSTRACT:

Background

The roles of p53 and HER2 in gastric adenocarcinoma (GAC) have been extensively studied; nevertheless, the contribution of mitochondrial transcription factor A (TFAM) remains unclear. Concerning TFAM's pivotal role in mitochondrial biogenesis, this study aimed to evaluate the TFAM expression in GAC and to assess its associations with p53 and HER2 expressions and clinicopathological outcomes.

Methods

We retrospectively analyzed 77 GAC patients who underwent upfront gastrectomy at Taipei Hospital between 2012 and 2021. Their clinicopathological profiles were recorded in detail. Immunohistochemical (IHC) staining for TFAM, p53, and HER2 protein expressions was semiquantified using IHC pixelwise H-score analyzed by ImageJ plugins IHC profiler. Associations between two

SUBMITTER: Ou LH 

PROVIDER: S-EPMC12707001 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature