Binary division of plastids involves endoplasmic reticulum mediation.

ABSTRACT: Plastids divide through binary division, involving the cytosolic protein Accumulation and Replication of Chloroplast 5 (ARC5), suggested to constrict and sever the plastid envelope membrane. However, the mechanisms involved in ARC5 recruitment to the mid-plastid division site and the final separation of daughter plastids are not fully understood. Using time-lapse imaging of Arabidopsis thaliana stable transgenics expressing fluorescently tagged endoplasmic reticulum (ER) and ARC5 proteins, we investigated the role played by the ER in the late stages of plastid division. Our observations establish that prior to its mid-plastid localization at the division plane, ARC5 associates with ER membranes. ARC5-ER association generates an ER band around the plastid mid-plane that persists throughout

SUBMITTER: Ghosh PP 

PROVIDER: S-EPMC12707056 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature