Development of Long-Term Human Adipocyte Organoids Manifesting Aging in Response to Intermittent Hypoxia.

ABSTRACT: Obstructive sleep apnea (OSA) (and consequent intermittent hypoxia (IH)) is increasingly recognized as a driver of adipose tissue dysfunction, insulin resistance, and aging. However, current in vitro experimental models inadequately capture the long-term effects of IH on human adipocytes. Here, we report the development and optimization of a robust long-term human adipocyte organoid culture system that faithfully recapitulates IH-induced adipocyte aging in vitro. Human stromal vascular fraction (SVF) cells, isolated from subcutaneous abdominal fat biopsies, were embedded in Matrigel and seeded into Biofloat U-bottom 96-well plates. Using a 1:1 Matrigel-cell mixture and optimized seeding volumes (5-20 μL), we established adipocyte organoids that formed within 10-12 days and re

SUBMITTER: Dong Y 

PROVIDER: S-EPMC12707283 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature