Protein Recognition of Glycosphingolipids in Membranes: Mechanistic and Quantitative Insights.

ABSTRACT: Recognition of glycosphingolipids (GSLs) in cell membranes by glycan-binding proteins (GBPs) is essential for diverse biological processes. However, owing to deficiencies in available analytical methods, the thermodynamics of GBP-GSL interactions remains poorly characterized. Native mass spectrometry (nMS) analysis performed using soluble GSL-containing model membranes provides a direct readout of the identity and stoichiometry of bound GSL ligands and, under certain conditions, can inform on affinity. Yet, for multivalent GBPs capable of engaging multiple model membranes simultaneously, data analysis relies on untested assumptions, which has limited adoption of the assay. Here, we apply mass photometry to quantify a series of high-affinity interactions between glycolipids in soluble model

SUBMITTER: Nguyen L 

PROVIDER: S-EPMC12707294 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature