Invasive properties of patient-derived glioblastoma cells after reversible electroporation <i>in vitro</i>.

ABSTRACT:

Background

Electroporation-based therapies are being explored in glioblastoma (GB) treatment, as means of enhancing drug delivery or achieving nonthermal ablation. Yet, little is known about how sublethal exposure affects the invasive behaviour of GB tumour cells.

Materials and methods

Five patient-derived GB cell lines were initially screened for intrinsic invasive potential, and two most invasive (NIB140 CORE and NIB216 CORE) were selected for further experiments with electroporation treatment. Cells in suspension were exposed to bursts of high-frequency biphasic electric pulses resulting in electric field strength of 1 kV/cm, which corresponded to conditions of reversible electroporation. Changes in cell invasion and gene regulation were assessed 24 hours after electropor

SUBMITTER: Blazic A 

PROVIDER: S-EPMC12707454 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature