Comparing Novel Low-Input Total RNA-Seq Methods.

ABSTRACT: Until recently, performing RNA-sequencing (RNA-seq) at low input often required specialized library preparation kits. The SMARTer® Stranded Total RNA-Seq Kit v3-Pico Input Mammalian workflow offered by Takara Bio is a popular option for generating high-quality libraries from low-input material. Recently, two RNA-seq library preparation kits, the Watchmaker RNA Library Prep Kit with Polaris Depletion by Watchmaker Genomics and the sparQ RNA-Seq HMR Kit by Quantabio, that offer a larger input range became available. These kits may offer a more accessible and affordable workflow for low-input RNA-seq; therefore, to determine their suitability for low-input applications, a comparative evaluation of the kits was performed using the market standard Takara Pico kit for benchmarking. To do this, t

SUBMITTER: Grimes B 

PROVIDER: S-EPMC12707489 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature