Improving Peak Capacity in Glycan Ion Mobility Separations through Traveling Wave-Based Ion Heating.

ABSTRACT: Complex carbohydrates, such as N-linked glycans, are highly important biomolecules with roles ranging from signaling to recognition and immune response. Ion mobility spectrometry-mass spectrometry (IMS-MS) has emerged as a rapid and orthogonal analytical technique to condensed-phase separations for studying carbohydrates, but many challenges exist in their analyses with IMS-MS due to their isomeric and conformational heterogeneity. Specifically, glycan IMS-MS separations often display more peaks than what can be predicted based on structure and/or much broader than expected peaks presumably from their metal-adducted conformers. This has precluded IMS-MS from being routinely used to analyze complex glycans largely because of the reduction in overall peak capacity and thus difficulty in deco

SUBMITTER: Rooney MR 

PROVIDER: S-EPMC12707584 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature