Optimizing the preparation of labeled N-glycans for rapid, simplified, and high-precision analysis.

ABSTRACT: Glycan structures hold promise as biomarkers for the early detection of diseases, owing to their sensitive reflection of cellular states. However, glycan analysis remains complex and time-consuming, and the use of hazardous chemicals in traditional hydrazinolysis methods presents a significant barrier to broader application and cross-disciplinary research. To enable efficient glycan biomarker discovery, we aimed to develop a simple and accurate N-glycan analysis method capable of high-throughput sample processing. A key feature of this study is the use of pyridylamination, a fluorescent labeling technique that enables high isomer separation efficiency in reversed-phase LC/MS. After comparing various methods for N-glycan release and purification, we identified Rapid PNGase F (New England Bi

SUBMITTER: Makino R 

PROVIDER: S-EPMC12707664 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature