Systematic characterization of mammalian extracellular vesicles using nano-flow cytometry.

ABSTRACT: Extracellular vesicles (EVs) are nanoscale, membrane-enclosed particles that transport bioactive cargo between cells and are increasingly studied for their potential in diagnostic and therapeutic applications. Advancing EV-based technologies for these applications depend on the ability to consistently isolate and characterize vesicle populations with defined biophysical and molecular properties. Efforts to obtain pure EV populations from cell culture systems are limited by inherent EV heterogeneity, exogenous particle contamination introduced by media supplements, and the co-isolation of non-vesicular contaminants. These challenges are further compounded by the limitations of conventional EV characterization platforms, which often lack the resolution to distinguish EVs from similarly sized

SUBMITTER: Vyzourek BT 

PROVIDER: S-EPMC12707723 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature