Anticancer Plant Secondary Metabolites Induce Linker Histone Depletion from Chromatin.

ABSTRACT:

Background

Many plant secondary metabolites (PSMs) were shown to intercalate into DNA helix or interact with DNA grooves. This may influence histone-DNA interactions changeing chromatin structure and genome functioning.

Methods

Nucleosome stability and linker histone H1.2, H1.4 and H1.5 localizations were studied in HeLa cells after the treatment with 15 PSMs, which are DNA-binders and possess anticancer activity according to published data. Chromatin remodeler CBL0137 was used as a control. Effects of PSMs were studied using fluorescent microscopy, flowcytometry, quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR), western-blotting.

Results

We showed that 1-hour treatment with CBL0137 strongly inhibited DNA synthesis and caused intensive linker his

SUBMITTER: Vlasova O 

PROVIDER: S-EPMC12707778 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature