Age-Related Changes in Myeloid Cells and Their Impact on Subcutaneous Melanoma Growth in Mice.

ABSTRACT:

Background

Advancing age increases cancer risk due to DNA damage accumulation and a decline in immune function. While aging is known to reduce adaptive immunity, it also leads to an increase in immunosuppressive myeloid cells, which promote tumor progression and are linked to poorer outcomes in melanoma.

Aims

This study aims to characterize how aging alters Gr-1⁺ myeloid cell function and their impact on melanoma growth and immunotherapy response.

Methods

Subcutaneous tumor growth using several YUMM melanoma cell lines was evaluated in 2-, 6-, and 12-month-old wild-type mice. Immune profiling of tumor-bearing and age-matched healthy mice was performed via flow cytometry and single-cell RNA sequencing. Gr-1⁺ myeloid cells were isolated to evaluate

SUBMITTER: Landreth KM 

PROVIDER: S-EPMC12707807 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature